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FlowIO is a specialized skill designed for handling Flow Cytometry Standard (FCS) files across versions 2.0 to 3.1. It enables researchers and developers to extract event data as NumPy arrays, access rich instrument metadata, and create new FCS files with minimal dependencies. This tool is essential for building scientific data pipelines, validating cytometry files, and performing pre-processing workflows before advanced analysis. By automating the extraction of scatter, fluorescence, and time-series data, it bridges the gap between raw biological instrument data and modern Python-based analysis libraries.