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Histolab simplifies the complex workflow of digital pathology by providing a robust framework for managing gigapixel whole slide images (WSI). It automates the detection of tissue regions, excludes background artifacts, and offers flexible tile extraction strategies—including random, grid, and score-based methods—to prepare high-quality datasets for machine learning. Designed for both exploratory analysis and production-grade pipelines, it supports multiple slide formats and provides extensive visualization tools to ensure accuracy at every step of the histological image processing chain.