Restriction Cloning

Give Claude your plasmid and insert (raw sequence, FASTA or GenBank) and it runs a tested restriction-mapping script instead of scanning sequences by eye, which is where language models quietly miss sites. What it does: • Restriction maps for ~40 common enzymes, or just the single- and non-cutters • Virtual digests with exact fragment sizes for linear or circular DNA, including sites that span the plasmid origin • Finds enzymes that cut your vector once (optionally inside the MCS) and don't cut your insert • Suggests directional enzyme pairs with incompatible ends • Golden Gate checks: finds internal BsaI, BsmBI, BbsI and SapI sites that need domesticating • Describes the expected gel, and flags bands too small to see or too close to resolve It also reminds you about Dam/Dcm methylation when you pick enzymes it can block (e.g. XbaI, ClaI). Reliable by design: pure Python standard library (nothing to install), and a built-in self-test checks cut positions, including Type IIS enzymes in both orientations, before use. Works in Claude Code and in Claude apps with code execution enabled.