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PyLabRobot is a powerful, hardware-agnostic SDK that enables the automation of laboratory workflows using Python. It provides a standardized way to control liquid handling robots like Hamilton STAR and Opentrons OT-2, along with analytical devices such as plate readers and heater shakers. With built-in support for resource tracking, deck layout management, and real-time 3D simulation, it allows scientists to develop and test reproducible protocols in a virtual environment before executing them on physical hardware, ensuring accuracy across Windows, macOS, and Linux platforms.