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PyLabRobot is a comprehensive Python SDK that allows developers and scientists to control laboratory hardware including Hamilton, Opentrons, and Tecan robots through a unified, hardware-independent API. It facilitates the creation of reproducible laboratory protocols, manages deck layouts and resources like plates and tips, and supports full-scale simulation before physical execution. By abstracting hardware-specific commands, it enables researchers to write protocols once and run them across different platforms, making it an essential tool for autonomous discovery and automated sample preparation.