Differential Methylation Analysis

소개

This skill streamlines the complex workflow of Whole Genome Bisulfite Sequencing (WGBS) analysis by automating the detection of Differential Methylation Regions (DMRs) and Differentially Methylated Cytosines (DMCs). It standardizes heterogeneous input files into a unified format, constructs merged methylation matrices, and leverages the metilene tool for statistical detection. By providing integrated filtering and visualization capabilities like volcano plots and length histograms, it allows researchers to rapidly interpret epigenetic differences between groups and generate exportable BED files for genome browser integration.

주요 기능

• Produces standardized output structures including BED files for genome browser visualization
• Automates the detection of both de-novo DMRs and site-specific DMCs
• Standardizes heterogeneous BED/BedGraph inputs into unified 4-column Metilene tables
• Generates quick visualizations including volcano plots and DMR length histograms
• Supports customizable filtering using q-value and delta methylation thresholds
• 2 GitHub stars

사용 사례

• Analyzing epigenetic differences between disease cases and control groups
• Identifying de-novo differentially methylated regions in WGBS datasets
• Processing and visualizing large-scale DNA methylation matrices for publication