Differential TAD Analysis

소개

This skill provides a structured workflow for identifying differentially interacting Topologically Associating Domains (TADs) between target and control samples. It automates complex bioinformatics tasks including Hi-C matrix normalization, TAD identification, and statistical comparison of intra- and inter-TAD interactions. By streamlining the use of HiCExplorer tools, it enables researchers to detect localized shifts in chromatin structure and generate high-quality visualizations of contact maps with overlaid TAD boundaries for comparative genomic studies.

주요 기능

• Generation of multi-track visualization files and Hi-C contact maps
• Comprehensive TAD identification with customizable window parameters
• Statistical analysis of intra- and inter-TAD interaction changes
• Automated Hi-C matrix normalization for comparable sequencing depth
• Support for .mcool, .cool, and .hic file format conversions
• 2 GitHub stars

사용 사례

• Comparing chromatin architecture between treated and control biological samples
• Detecting structural domain changes across different cell types or lineages
• Identifying localized genomic structural shifts following experimental perturbations