Qpcr Analysis

Paste your qPCR export and Claude runs a tested analysis script instead of doing ΔΔCt arithmetic in its head. What it does: • Relative expression by 2^-ΔΔCt, or efficiency-corrected (Pfaffl / qbase) when you give primer efficiencies • One or several reference genes, combined by geometric mean • Averages technical replicates and flags any with SD above 0.5 Ct • Warns when a reference gene's Ct drifts more than 2 cycles across samples • Drops 'Undetermined' wells and tells you which ones • Group summaries: fold change and log2FC ± SD per target and condition • Primer efficiency, slope and R² from a standard-curve dilution series Claude maps QuantStudio, CFX or LightCycler exports to the expected format and shows you which columns it used, and reminds you to run statistics on log2 values, not fold changes. Reliable by design: pure Python standard library (nothing to install), and a built-in self-test checks the math against Livak & Schmittgen before use. Works in Claude Code and in Claude apps with code execution enabled.