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Histolab is a specialized toolkit for researchers and developers working with large-scale histological images. It automates the complex workflow of handling gigapixel Whole Slide Images (WSI), allowing users to accurately detect tissue regions, exclude artifacts, and extract informative tiles using various strategies like random, grid, or score-based sampling. By streamlining the preprocessing phase, it facilitates the creation of high-quality datasets for computational pathology and machine learning models, ensuring that downstream analysis focuses on the most relevant biological data.