Protein Assembly

关于

Streamlines the complex process of engineering fusion proteins by providing a systematic framework for component identification, sequence extraction, and structural assembly. This skill offers specialized guidance for matching fluorescent proteins based on spectral properties, selecting appropriate linkers, and performing codon optimization with rigorous GC content constraints, ensuring that generated sequences are ready for laboratory synthesis and functional validation.

主要功能

• Sequence extraction and validation from PDB, FPbase, and GenBank sources
• Multi-domain assembly with intelligent linker selection and N-terminal handling
• Codon optimization for target organisms with sliding-window GC validation
• 16 GitHub stars
• Standardized verification checkpoints for FRET compatibility and synthesis limits
• Systematic identification of proteins via spectral properties and database queries

使用场景

• Generating optimized gBlock sequences for commercial gene synthesis
• Constructing FRET-based biosensors with precise spectral matching
• Designing tagged protein constructs for specialized binding applications