Protein Assembly

关于

This skill provides a comprehensive framework for bioengineers and researchers to streamline the design of complex fusion protein gBlock sequences. It guides the assembly of multi-domain proteins sourced from PDB, plasmid files, and fluorescent protein databases, handling technical nuances such as N-terminal methionine removal and exact spectral wavelength matching. By enforcing a structured six-phase workflow—from initial cataloging to sliding-window GC content optimization—the skill ensures the resulting DNA constructs are valid, continuous, and optimized for laboratory synthesis.

主要功能

• Automated sequence retrieval and cataloging from PDB, GenBank, and protein databases
• Sliding-window GC content optimization using synonymous codon swapping
• Standardized N-terminal methionine handling and flexible GS linker design
• 16 GitHub stars
• Comprehensive verification checkpoints for sequence order, length, and synthesis constraints
• Precise matching for fluorescent proteins based on exact excitation and emission wavelengths

使用场景

• Assembling fluorescent reporter constructs with specific spectral properties for imaging
• Designing complex multi-domain fusion proteins for synthetic biology research
• Optimizing DNA sequences for commercial gBlock synthesis with strict GC content requirements